PHYTOCHEMICAL AND ANTIMICROBIAL SCREENING OF THE LEAF AND ROOT OF ALBIZIA LEBBEK (SUGAR TARA)

AUTHOR: LACKSON ALBERT HELWA

DEPARTMENT: PURE AND INDUSTRIAL CHEMISTRY

AFFILIATION: NNAMDI AZIKIWE UNIVERSITY, AWKA

The preliminary phytochemical screening, extraction and determination of the active compounds present and antimicrobial tests were carried out on the leaf and root of Albizia lebbek. The leaf and root extracts of the plant reveal the presence of alkaloids, steroids, tannins, glycosides, flavonoids and saponins. The alkaloid and other fractions of the leaf and root crude drugs were extracted by standard methods. The extracts were purified by use of thin layer chromatography (TLC) and PTLC. TLC revealed the number of compounds present and their Rf values were calculated. The Fourier Transform Infrared (FTIR) and UV-VIS were used to characterize the leaf and root extracts. The FTIR showed the presence of the following functional groups: -C=S, OH, N-H, RC=CR, C=N, C-N, C-H, -C=O,C-O-C, P-H, CO, and the UV-VIS showed the presence of  aromatic carbonyl group, unsaturated carbonyl compounds, phenols, ethylenic group, the presence of nitrogen containing monocyclic aromatic compounds and highly unsaturated system in the compound. The antimicrobial activity of the plant’s leaf and root extracts was assayed and showed that the extracts were effective on some test organisms. There was no significant antifungal activity on the leaves. The test organisms were Escherichia coli, Staphylococcus aureus, Klebsiella auerogenes, Proteus vulgaris, Bacillus specie, Streptococcus specie, Enterobacter aerogenes, Pseudomonas pyocyania, Aspergillus niger, Aspergillus  flavus, and Candida albicans. This study has justified the traditional use of this plant for the treatment of cough, infertility, hypertension, etc. also a remedy for wound healing whose causative agents are some of the organisms used in this work.

TO VIEW THE FULL CONTENT OF THIS DOCUMENT, PLEASE VISIT THE UNIZIK LIBRARY WEBSITE USING THIS LINK, http://naulibrary.org/dglibrary/admin/book_directory/Thesis/11079.pdf

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