BACTERIAL UTILIZATION OF LINEAR ALKYLBENZENESULFONATES DETERGENTS

AUTHOR: NNAEBUE KENNETH CHUKWUGOZIE

DEPARTMENT: APPLIED MICROBIOLOGY AND BREWING

AFFILIATION: NNAMDI AZIKIWE UNIVERSITY, AWKA

Biodegradability of four powdered commercial detergent products with trade names “Omo”, “Ariel”, “Klin” and “Zip” by indigenous bacteria isolated from Ofifia stream, Unizik stream, Cilia’s kitchen sullage and Primerose Laundry grey water was investigated. The detergent utilizing bacteria were identified as Pseudomonas aeruginosa, Bacillus subtilis, Pantoeaagglomerans, Staphylococcus epidermidis, and Escherichia coli with mean heterotrophic count of 1 x 105cfu/ml. Screening test with mineral salt detergent medium revealed that Pseudomonas aeruginosa showed the highest utilizing ability while the least was shown by Escherichia coli. Chemical changes monitored showed that pH shifted from alkaline towards acidic range 6.6, 6.1 6.4 and 6.3 forOmo, Ariel, Klin and Zip respectively in mixed culture and 6.9, 6.4, 6.6, and 6.1 for Omo, Zip, Klin and Ariel respectively in single culture by bacterial metabolism as a result of production of acidic metabolites. Progressive degradation of the detergents at 0.01 %( w/v) using single culture and mixed culture was monitored. Methylene blue active substance ranges of 0.19 – 0.32 mgl-1 and 0.13 – 0.21 mgl-1 for single and mixed culture respectively were obtained in the study. Statistical analysis using unpaired t-test showed that there was significant difference between the mean values obtained from mixed culture and those obtained from single culture at P < 0.05. Percentage degradation values revealed that all the specimen studied were above 80% at day 24 which is the European Union minimum biodegradability regulatory requirement in packaged detergents except Omo which showed 79% and 68% in mixed and single cultures respectively. This shows that some detergents made in Nigeria still contain components that are not easily degraded by natural bacterial flora.

TO VIEW THE FULL CONTENT OF THIS DOCUMENT, PLEASE VISIT THE UNIZIK LIBRARY WEBSITE USING THIS LINK, http://naulibrary.org/dglibrary/admin/book_directory/Thesis/11618.pdf

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