MYCO-UTILIZATION OF CASSAVA WASTE (PEELS)

AUTHOR: EMEH GRACE ONYEKA

DEPARTMENT: APPLIED MICROBIOLOGY AND BREWING

AFFILIATION: NNAMDI AZIKIWE UNIVERSITY, AWKA

The growth and bioconversion potentials of selected species of fungi growing on varieties of cassava waste the peels (TMS/0581, TME/419 and TMS/30572) during solid state fermentation were assessed. The myco-utilization of cassava waste were carried out by evaluating pretreated cassava waste and untreated cassava waste in solid state fermentation with mixed culture of Aspergillus terrus and Aspergillus niger, single culture of Aspergilllus terreus and Aspergillus niger. The result revealed that the interaction between the mixed culture of Aspergillus niger and Aspergillus terrus and cassava variety TMS/0581 of treated cassava waste yielded better performance in protein enhancement and reducing sugar production than the single culture and other cassava varieties. The mixed culture showed 86.6%, 82.4% and 80.3% starch saccharification for cassava waste TMS/0581, TME/419 and TMS/30572 respectively for the pretreated waste and releasing 60.7%, 53.2% and 41.7% reducing sugar, the protein content gradually increased from 2.5% to 14.4%, 10.2% and 14% respectively on 6th day of the fermentation. For untreated cassava waste, the mixed culture showed 75.5%, 74.9% and 75.2% starch saccharification for TMS/0581, TME/419 and TMS/30572 respectively releasing 31.1%, 29.1% and 28.8% reducing sugar , the protein content gradually increased from 2.5% to 9.5%, 8.8% and 10.2% respectively on 9th day of the solid state fermentation. The amylase activity of the different culture was monitored and the highest amylase activity was observed at the peak of fermentations. Statistical analysis using paired t – test showed that there is significant difference between the mean values obtained from pretreated cassava waste and untreated cassava waste P < 0.05. This study demonstrated the feasibility of bioconversion of cassava waste into value – added products.

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