SCREENING OF POTASH COMPOUNDS FOR TREATMENT OF CUTANEOUS CANDIDIASIS USING ALBINO MICE MODEL

AUTHOR: UDEMEZUE, ONYEKACHUKWU IZUCHUKWU

DEPARTMENT: APPLIED MICROBIOLOGY AND BREWING

AFFILIATION: NNAMDI AZIKIWE UNIVERSITY, AWKA

In order to ascertain the efficacy of some of commonly used toothpastes in Nigeria and to improve their antimicrobial qualities twenty three 23 toothpaste users were enrolled in the study. Fifty (50) samples were collected of which 50% were mouth swabs and 50% were mouth washes. Toothpaste (TP) C had the highest number of users and samples collected from users constituted 24% of the total samples. Hundred isolates were obtained and characterized of which percentage of occurrence ranged from 22% of S. aureus to 4% of C. albicans. pH of toothpastes was determined and the mean(±STD) pH of toothpastes ranged from 6.47±0.07 of TP E to 7.70±0.07 of TP B. The MIC and MBC of antimicrobial agents on isolates was determined by broth dilution methods and ranged from 0.04mg/ml and 0.625mg/ml of Ciprofloxacin to 3.75 and 5.0mg/ml of Erythromycin. The Kirby-Bauer Disc diffusion method was used to determine antimicrobial activities of the toothpastes in vitro. All toothpastes showed antimicrobial activity on isolates with mean zone of inhibition ranging from 11-36mm. E. coli showed the highest sensitivity of 18% while C. albicans showed lowest sensitivity of 13.2% to toothpastes. TP A and TP F showed the highest effects on the isolates. Crude extracts of C. limon, C nucifera, A. occidentale and D tripetla were obtained and screened in vitro for antimicrobial activities against isolates. S. mutans showed highest overall sensitivity of 22.9% to all the extracts. A new toothpaste (FennaPaste) was formulated with common ingredients seen on toothpastes’ packs and also screened in vitro for antimicrobial activities against isolates. There was significant difference in the activity of conventional toothpastes and newly formulated toothpaste p>0.05 when extracts obtained were added into the toothpastes. Modifications of the extracts by heating and increasing of pH to 8.0 gave significant difference in activity p>0.05 Addition of these extracts in toothpaste is therefore suggested for improved antimicrobial effects.

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