STUDIES ON ANTIBACTERIAL ACTIVITIES OF LACTOBACILLUS ACIDOPHILUS ISOLATED FROM AKAMU ON MICE MODEL SHIGELLOSIS

Author: Ani Samuel Chukwunonso
Department: Applied Microbiology
Affiliation: Nnamdi Azikiwe University Awka

Lactobacillus acidophilus was isolated from samples of akamu purchased in Nsukka main market. The lactic acid bacterium and the pathogenic Shigella dysenteriae were identified to specie level using 16s rRNA analysis and also through their morphological appearances, and some biochemical tests. The Lactobacillus acidophilus showed resistance to bile, acidity, growth at 6.5% NaCl solution, positive cell surface hydrophobicity test and antibacterial activity against Shigella dysenteriae. However, the Lactobacillus acidophilus prophylactic and curative activities against intestinal bacillary dysenteriae were demonstrated using 50 freshly weaned mice that were divided into the following groups: A (undiseased), B (diseased without treatment), C (diseased and treated with ampicillin), D (diseased and treated with Lactobacillus acidophilus.), and E (prophylactic group). Mice groups were subjected to same environmental conditions. The organism (Lactobacillus acidophilus) showed a strong prophylactic and curative activity against Shigella dysenteriae in vivo. The packed cell volume amongst the mice groups was significantly different with p 0.05. Differential blood count showed neutrophils and lymphocytes had significant difference with P 0.05. Histological analysis was performed on the intestinal tissue with Haematoxylin – Eosin stain and it showed the presence of neutrophils in the mucosal and submucosal layer of the intestine. Microabscess was evident on the intestinal walls for groups B, C, D and there was no corresponding evidence of microabcess with mucosa which depicts normal intestinal wall organization for groups A and E.

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