ANTIMICROBIAL SUSCEPTIBILITY AND PLASMID PROFILE OF Staphylococcus aureus ISOLATED FROM DIFFERENT CLINICAL SPECIMENS OF PATIENTS ATTENDING GENERAL HOSPITAL IN ONITSHA

Author: Izundu Ijeoma Monica
Department: Applied Microbiology
Affiliation: Nnamdi Azikiwe University

The resistance profile of microorganisms, especially Staphylococcus aureus to antibiotics is increasing because of several factors including the widespread use of antibiotics. In this study, 400 specimens made up of 200 urine samples, 50 HVS (high vaginal swab), 50 urethral swab, 50 wound swab and 50 semen samples were collected from patients attending clinics at General Hospital Onitsha, in Anambra State, South East Nigeria. The samples were examined of the presence of Staphylococcus aureus macroscopically by observing the color and the size, microscopically using gram’s staining and biochemically using catalase test, coagulase test and by culturing on manitol agar. The antibiotics sensitivity of the isolates was determined using disk diffusion method. The resistant strains were screened for the presence of R plasmid. The result showed that for urine samples, there were 49( 12.25%), for HVS 10(2.5%) isolates, for urethral swab there were 5 (1.25%), for semen there were 3 (0.75%) and for wound swab there were 3(0.75%) isolates. The antibiotics susceptibility profile showed that Staphylococcus aureus was highly susceptible to the antibiotics as follows: levefloxacin (70%) followed by Ciprofloxacin (65.71%) and Ofloxacin (64.29%) while 80% was resistant to Augumentin and 57.43%, 54.29% were resistant to lincomycin and Erythromycin respectively. The result showed that plasmids ranging from 2.185kbp to 20.239kbp were harboured by the Staphylococcus aureus and that most of the resistant plasmids were cureable.

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