Author: Nnaebue Ndirika Doris
Department: Applied Microbiology
Affiliation: Nnamdi Azikiwe University Awka

The prevalence of human pathogenic bacteria especially Salmonella species in cow dung was investigated. Five different abattoirs in Anambra State was used. Eight different potentially pathogenic organisms were isolated and identified and they include; Salmonella enteritidis, Shigella sp., Clostridium perfringens., Pseudomonas sp., E. coli, Staphylococcus aureus, Vibrio cholerae and Vibrio parahemolyticus. The susceptibility of the isolates to different antibiotics was tested and it was observed that Pseudomonas sp. is very sensitive to the antibiotics, Ceporex (10μg) and Tarivd (10μg) with 16mm zone of inhibition. Clostridium sp. is more susceptible to Levofloxacin (20μg) with 20mm zone of inhibition, Staph. aureus is more susceptible to Streptomycin (30μg) and Levoflaxacin (20μg) with 18mm zone of inhibition, E. coli is more susceptible to Gentamycin (10μg) with 20mm zone of inhibition, Salmonella enteritidis is more sensitive to Augmentin (30μg) with 20mm zone of inhibition and Shigella sp. is more sensitive to Ciproflox (10μg) with 20mm zone of inhibition. The pathogenicity of these isolates were studied by infecting each on mice. There was death of two mice infected with Clostridium perfringens .On the mice infected with Shigella sp., there was swollen of the scrotum and scrotal sac which was observed after the dissection. On the other mice infected with Pseudomonas sp., Staphylococcus aureus, Salmonella enteritidis. and E. coli, 25×108, 8×108, 20×108, 10×108 cfu/ml of the infected organisms were recovered from their intestine respectively. This shows that the organisms colonized their intestine at high level and they shed them in their faeces, though the infections were symptomless.

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