Author: Poopola Omolara Dorcas
Department: Applied Microbiology
Affiliation: Nnamdi Azikiwe University Awka
This study was aimed at determining the prevalence and intensity of urinary schistosomiasis and other urinary tract infections among school children in Ijebu North L.G.A, Ogun State, Nigeria. Three hundred and seventy-two urine samples were randomly collected from primary school children (one hundred and eighty-six samples each from both sexes) and analysed using standard microbiological procedures. Results of this study showed that, none of the examined subjects had urinary schistosomiasis. However, an elevated trend of bacterial flora were isolated as follows; Escherichia coli 49.4% (88 male), 49.5% (96 female), Pseudomonas aeroginosa 18.5% (33 male), 18.6% (36 female), Klebsiella pneumoniae 16.9% (30 male), 17.0% (33 female), Proteus mirabilis 6.7% (12 male), 7.2% (14 female), Serratia marcensens 3.4% (6 male), 3.1% (6 female), Enterobacter aerogenes 5.1% (9 male), 4.6% (9 female) for Gram negative organisms while the Gram positive ones were Staphylococcus aureus 55.3% (78 male), 60.6% (140 female), Staphylococcus saprophyticus 24.1% (34 male), 20.8% (48 female), Enterococcus faecalis 13.5% (19 male), 14.3% (33 female), and Bacillus subtilis 7.1% (10 male), 4.3% (10 female). Pseudomonas aeroginosa, Proteus mirabilis and Escherichia coli showed 100% resistance to Augmentin (30μg), Ofloxacin (5μg), Gentamicin (10μg), Nalixidic acid (30μg), Nitrofuratoin (200μg), Cotrimoxazole (25μg), Amoxycillin (25μg), Tetracycline (25μg), while the strains of Klebsiella pneumoniae isolated were highly susceptible to the aforementioned antibiotics except for tetracycline (100%). Of the Gram positive, Staphylococcus aureus was found to be the most resistant to 25μg Cotrimoxazole (0mm), 5μg Cloxacillin (0mm), 5μg Erythromycin (0mm), 10μg Gentamicin (15mm), 30μg Augmentin (12mm), 10μg Streptomycin (15mm), 10μg Tetracycline (0mm), 10μg Chloramphenicol (15mm); followed by Bacillus subtilis, Staphylococcus saprophyticus, and Enterococcus faecalis. Also, Candida albicans 60% (6 male), 62.5% (10 female) and other Candida species 40% (4 male), 37.5% (6 female) were isolated and identified after carrying out a germ tube test. Gram negative organisms were found harboring efflux pump resistant system after the incorporation of an efflux inhibitor, carbonyl cyanide m-chlorophenyl hydrazone into the MIC (minimum inhibitory concentration) medium.
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Tags: Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, Nnamdi Azikiwe University, Popoola Omolara Dorcas, Proteus mirabilis, Pseudomonas aeruginosa, Schistosomiasis, Serratia marcensens, Uninary Tract Infection