Archive for the ‘Faculty of Basic Medical Science’ Category

The Effects of BROMOCRIPTINE and METOCLOPRAMIDE on Lipid Profile in Male Albino Rats

June 3, 2016

Author: Oparaji Chiedozie Kenneth
Department: Human Physiology
Affiliation: Nnamdi Azikiwe University Awka

The metabolic effects of serum prolactin perturbations are often overlooked. Recent data indicates a role for the prolactin hormone as a biomarker of cardiovascular risks. To study the effects of Bromocriptine and Metoclopramide on Lipid Profile, 40 male albino rats weighing between 150-180 grams were used. They were distributed into four groups of 10 rats each. Group I served as the negative control and were fed normal pelletized growers feed. Group II
served as the positive control and were fed a diet comprising normal rat feed mixed with 2.4% bleached palm oil extract. Group III received the same fat diet and a daily oral dose of 5mg/kg of the hypoprolactinaemic drug, Bromocriptine (Bromergon, Slovenia). Group IV also received the fat feed and were treated with a daily dose of 20mg/kg of Metoclopramide (Mederax, China), administered intraperitoneally to induce hyperprolactinemia.The animals were acclimatized for 14 days while treatment lasted for another 14 days. After treatment, their blood samples were
collected into sterile sample containers and the serum Prolactin (PRL) concentration was determined using ELISA Well Washer Stat Fax 2600 (Aware Technology Inc., USA) and ELISA Reader Stat Fax 2100 (Aware Technology Inc, USA). The Lipid Profile parameters – Total Cholesterol (TC), Triglycerides (TG), High Density Lipoproteins (HDL), and Low Density Lipoproteins – were determined usingTRSP-722 Spectrophotometer (TRIUP International corporation, China). There was a statistically significant difference (P0.05), the bromocriptine-treated rats showed lower mean TC, TG and LDL values as well as lower lipid ratio and atherogenic indices when compared to the positive control animals and the metoclopramide treated rats. The metoclopramide-treated rats showed the highest lipid values as well as the highest lipid ratio and atherogenic indices. Metoclopramide-induced hyperprolactinemia was associated with higher lipid values while bromocriptine-induced hypoprolactinaemia was associated with lower concentration of the lipid profile parameters. In summary, manipulation of endogenous PRL concentration can lead to hyperlipidaemia and thus contribute to an atherogenic phenotype.

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The Effects of Metoclopramide and Bromocriptine on Immune-related Leucocyte of Dogs

June 3, 2016

Author: Obinwogu Donatus Lotanna
Department: Human Physiology
Affiliation: Nnamdi Azikiwe University Awka

The relationship between the prolactin hormone and immunity has been variously established. In the present study, Metopclopramide and Bromocriptine were used to induce hyperprolactinaemia and hypoprolactinaemia, respectively, to evaluate the effects on immune related leucocytes of dogs. Thirty male Mongrel dogs weighing between 5kg and 10kg were used for the study. The experiment lasted for 28 days. The dogs were acclimatized for the first 14 days after which metopclopramide and bromocriptine were administered. The animals were split into ten dogs per group. Group 1 served as the control and was given distilled water. Group 2 was administered 20mg metoclopramide injection intramuscularly per body weight and served as the hyperprolactinaemic group. Group 3 was administered with an oral daily dose of 5mg of bromocriptine per body weight which served as hypopolactinaemic group. The result of the study showed that there was a statistically significant increase in prolactin levels (10.66±0.92ng/ml) of the hyperprolactinaemic group compared to the control group (9.95±0.56), (P<0.05). Also, the mean total white blood cell count of the hyperprolactinaemic group (524.00±203.63) showed a statistically significant difference (P=0.02) when compared to the control (345±82.41). Comparison between the total white blood cell count of the hyperprolactinaemic group (524.00±203.63) and the hypoprolactinaemic group (321.80±124.78) also showed a statistical significance (P=0.01). The finding of this study suggests that hyperprolactinaemia might be associated with an increase in leucocyte profile.

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The Effects of Metoclopramide and Bromocriptine on Immune – related Leucocyte of Dogs

March 10, 2016

Author: OBINWOGU DONATUS LOTANNA

Department: HUMAN PHYSIOLOGY

Affiliation: Nnamdi Azikiwe University, Awka

The relationship between the prolactin hormone and immunity has been variously established. In the present study, Metopclopramide and Bromocriptine were used to induce hyperprolactinaemia and hypo
prolactinaemia, respectively, to evaluate the effects on immune related leucocytes of dogs. Thirty male Mongrel dogs weighing between 5kg and 10kg were used for the study. The experiment lasted for 28 days. The dogs were acclimatized for the first 14 days after which metopclopramide and bromocriptine were administered. The animals were split into ten dogs per group. Group 1 served as the control and was given distilled water. Group 2 was administered 20mg metoclopramide injection intramuscularly per body weight and served as the hyper prolactinaemic group. Group 3 was administered with an oral daily dose of 5mg of bromocriptine per body weight which served as hypopolactinaemic group. The result of the study showed that there was a statistically significant increase in prolactin levels (10.66 ± 0.92ng/ml) of the hyper prolactinaemic group compare d to the control group (9.95 ± 0.56) (P < 0.05) . Also, the mean total white
blood cell count of the hyperprolactinaemic group (524.00 ± 203.63) showed a statistically significant
difference (P=0.02) when compared to the control (345 ± 82.41). Comparison between the total white blood cell count of the hyperprolactinaemic group (524.00 ± 203.63) and the hypoprolactinaemic group (321.80 ±
124.78) also showed a statistical significance (P=0.01). The finding of this study suggests that
hyperprolactinaemi a might be associated with an increase in leucocyte profile.

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